DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters.

ABSTRACT: A bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C(3)AC(3)AC(3)GC(3)A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nanomolar concentrations using diode laser excitation. Pendant to this cluster encoding region, the recognition component binds a target DNA strand through hybridization, and decoupling of these two regions of the composite sensor renders a modular sensor for specific oligonucleotides. A target is detected using a quencher strand that bridges the cluster template and recognition components and disturbs cluster binding, as indicated by static quenching. Competitive displacement of the quencher by the

SUBMITTER: Petty JT 

PROVIDER: S-EPMC4201625 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature