Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates.

ABSTRACT:

Background

Genome-wide profiling of single-nucleotide polymorphisms is receiving increasing attention as a method of pre-implantation genetic diagnosis in humans and of commercial genotyping of pre-transfer embryos in cattle. However, the very small quantity of genomic DNA in biopsy material from early embryos poses daunting technical challenges. A reliable whole-genome amplification (WGA) procedure would greatly facilitate the procedure.

Results

Several PCR-based and non-PCR based WGA technologies, namely multiple displacement amplification, quasi-random primed library synthesis followed by PCR, ligation-mediated PCR, and single-primer isothermal amplification were tested in combination with different DNA extractions protocols for various quantities of genomic DNA inputs. T

SUBMITTER: Shojaei Saadi HA 

PROVIDER: S-EPMC4201692 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature