Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells.

ABSTRACT: High throughput detection of differential expression of genes is an efficient means of identifying genes and pathways that may play a role in biological systems under certain experimental conditions. There exist a variety of approaches that could be used to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a paramyxovirus. SSH was used for initial isolation of differentially expressed transcripts, a large-scale validation of which was accomplished by microarray analysis. The data reveals a large group of regu

SUBMITTER: Munir S 

PROVIDER: S-EPMC420231 | biostudies-literature | 2004

REPOSITORIES: biostudies-literature