Immunodominant T-cell epitopes of MOG reside in its transmembrane and cytoplasmic domains in EAE.

ABSTRACT:

Objective

Studies evaluating T-cell recognition of myelin oligodendrocyte glycoprotein (MOG) in multiple sclerosis (MS) and its model, experimental autoimmune encephalomyelitis (EAE), have focused mostly on its 117 amino acid (aa) extracellular domain, especially peptide (p) 35-55. We characterized T-cell responses to the entire 218 aa MOG sequence, including its transmembrane and cytoplasmic domains.

Methods

T-cell recognition in mice was examined using overlapping peptides and intact full-length mouse MOG. EAE was evaluated by peptide immunization and by adoptive transfer of MOG epitope-specific T cells. Frequency of epitope-specific T cells was examined by ELISPOT.

Results

Three T-cell determinants of MOG were discovered in its transmembrane and cytoplasmic domains

SUBMITTER: Shetty A 

PROVIDER: S-EPMC4202928 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature