Identifying species of moths (Lepidoptera) from Baihua Mountain, Beijing, China, using DNA barcodes.

ABSTRACT: DNA barcoding has become a promising means for the identification of organisms of all life-history stages. Currently, distance-based and tree-based methods are most widely used to define species boundaries and uncover cryptic species. However, there is no universal threshold of genetic distance values that can be used to distinguish taxonomic groups. Alternatively, DNA barcoding can deploy a "character-based" method, whereby species are identified through the discrete nucleotide substitutions. Our research focuses on the delimitation of moth species using DNA-barcoding methods. We analyzed 393 Lepidopteran specimens belonging to 80 morphologically recognized species with a standard cytochrome c oxidase subunit I (COI) sequencing approach, and deployed tree-based, distance-based, and diagno

SUBMITTER: Liu XF 

PROVIDER: S-EPMC4203292 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature