Dataset Information

A novel Azotobacter vinellandii (SRIAz3) functions in salinity stress tolerance in rice.

ABSTRACT: The plant growth promoting rhizobacteria (PGPRs) as a biofertilizer provide agricultural benefits to advance various crops productivity. Recently, we discovered a novel Azotobacter vinellandii (SRIAz3) from rice rhizosphere, which is well competent to improve rice productivity. In this study, we investigated a role of A. vinellandii to confer salinity tolerance in rice (var. IR64). A. vinellandii inoculated rice plants showed higher proline and malondialdehyde content under 200 mM NaCl stress as compared with uninoculated one. The endogenous level of plant hormones viz., indole-3 acetic acid (IAA), gibberellins (GA3), zeatint (Zt) was higher in A. vinellandii inoculated plants under high salinity. The fresh biomass of root and shoot were relatively elevated in A. vinellandii inoculated rice. Further, the macronutrient profile was superior in A. vinellandii inoculated plants under salinity as compared with non-inoculated plants. The present findings further suggest that A. vinellandii, a potent biofertilzer, potentially confer salinity stress tolerance in rice via sustaining growth and improving compatible solutes and nutrients profile and thereby crop improvement.

SUBMITTER: Sahoo RK

PROVIDER: S-EPMC4203646 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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A novel Azotobacter vinellandii (SRIAz3) functions in salinity stress tolerance in rice.

Sahoo Ranjan Kumar RK Ansari Mohammad Wahid MW Pradhan Madhusmita M Dangar Tushar K TK Mohanty Santanu S Tuteja Narendra N

Plant signaling & behavior 20140101 7

The plant growth promoting rhizobacteria (PGPRs) as a biofertilizer provide agricultural benefits to advance various crops productivity. Recently, we discovered a novel Azotobacter vinellandii (SRIAz3) from rice rhizosphere, which is well competent to improve rice productivity. In this study, we investigated a role of A. vinellandii to confer salinity tolerance in rice (var. IR64). A. vinellandii inoculated rice plants showed higher proline and malondialdehyde content under 200 mM NaCl stress as ...[more]

PMID: 25763502

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Project description:Background and aimsAgricultural productivity is increasingly being affected by the build-up of salinity in soils and water worldwide. The genetic base of salt-tolerant rice donors being used in breeding is relatively narrow and needs broadening to breed varieties with wider adaptation to salt-affected areas. This study evaluated a large set of rice accessions of diverse origins to identify and characterize novel sources of salt tolerance.MethodsDiversity analysis was performed on 107 germplasm accessions using a genome-wide set of 376 single-nucleotide polymorphism (SNP) markers, along with characterization of allelic diversity at the major quantitative trait locus Saltol Sixty-nine accessions were further evaluated for physiological traits likely associated with responses to salt stress during the seedling stage.Key resultsThree major clusters corresponding to the indica, aus and aromatic subgroups were identified. The largest group was indica, with the salt-tolerant Pokkali accessions in one sub-cluster, while a set of Bangladeshi landraces, including Akundi, Ashfal, Capsule, Chikirampatnai and Kutipatnai, were in a different sub-cluster. A distinct aus group close to indica contained the salt-tolerant landrace Kalarata, while a separate aromatic group closer to japonica rice contained a number of traditional, but salt-sensitive Bangladeshi landraces. These accessions have different alleles at the Saltol locus. Seven landraces - Akundi, Ashfal, Capsule, Chikirampatnai, Jatai Balam, Kalarata and Kutipatnai - accumulated less Na and relatively more K, maintaining a lower Na/K ratio in leaves. They effectively limit sodium transport to the shoot.ConclusionsNew salt-tolerant landraces were identified that are genetically and physiologically distinct from known donors. These landraces can be used to develop better salt-tolerant varieties and could provide new sources of quantitative trait loci/alleles for salt tolerance for use in molecular breeding. The diversity observed within this set and in other donors suggests multiple mechanisms that can be combined for higher salt tolerance.

Project description:Rice tolerance to salinity stress involves diverse and complementary mechanisms, such as the regulation of genome expression, activation of specific ion-transport systems to manage excess sodium at the cell or plant level, and anatomical changes that avoid sodium penetration into the inner tissues of the plant. These complementary mechanisms can act synergistically to improve salinity tolerance in the plant, which is then interesting in breeding programs to pyramidize complementary QTLs (quantitative trait loci), to improve salinity stress tolerance of the plant at different developmental stages and in different environments. This approach presupposes the identification of salinity tolerance QTLs associated with different mechanisms involved in salinity tolerance, which requires the greatest possible genetic diversity to be explored. To contribute to this goal, we screened an original panel of 179 Vietnamese rice landraces genotyped with 21,623 SNP markers for salinity stress tolerance under 100 mM NaCl treatment, at the seedling stage, with the aim of identifying new QTLs involved in the salinity stress tolerance via a genome-wide association study (GWAS). Nine salinity tolerance-related traits, including the salt injury score, chlorophyll and water content, and K+ and Na+ contents were measured in leaves. GWAS analysis allowed the identification of 26 QTLs. Interestingly, ten of them were associated with several different traits, which indicates that these QTLs act pleiotropically to control the different levels of plant responses to salinity stress. Twenty-one identified QTLs colocalized with known QTLs. Several genes within these QTLs have functions related to salinity stress tolerance and are mainly involved in gene regulation, signal transduction or hormone signaling. Our study provides promising QTLs for breeding programs to enhance salinity tolerance and identifies candidate genes that should be further functionally studied to better understand salinity tolerance mechanisms in rice.

Dataset Information

A novel Azotobacter vinellandii (SRIAz3) functions in salinity stress tolerance in rice.

Publications

A novel Azotobacter vinellandii (SRIAz3) functions in salinity stress tolerance in rice.

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