Dried fluid spots for peste des petits ruminants virus load evaluation allowing for non-invasive diagnosis and genotyping.

ABSTRACT:

Background

Active surveillance of peste des petits ruminants (PPR) should ease prevention and control of this disease widely present across Africa, Middle East, central and southern Asia. PPR is now present in Turkey at the gateway to the European Union. In Bangladesh, the diagnosis and genotyping of PPR virus (PPRV) may be hampered by inadequate infrastructures and by lack of proper clinical material, which is often not preserved under cold chain up to laboratories. It has been shown previously that Whatman® 3MM filter paper (GE Healthcare, France) preserves the nucleic acid of PPRV for at least 3 months at 32°C.

Results

In this study, we demonstrate the performances of filter papers for archiving RNA from local PPRV field isolates for further molecular detection and genoty

SUBMITTER: Bhuiyan AR 

PROVIDER: S-EPMC4203889 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature