Suitability of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp.

ABSTRACT:

Background

The purpose of this study was to assess the genetic variation and differentiation of Blastocystis subtypes (STs) recovered from symptomatic children by analysing partial sequences of the small subunit rDNA gene region (SSUrDNA) and internal transcribed spacers (1 and 2) plus the 5.8S region (ITS, ITS1 + 5.8S + ITS2) and comparing with isolates from other countries.

Findings

Faecal samples from 47 Blastocystis-infected children with gastrointestinal symptoms and negative for pathogenic enterobacteria were analysed. PCR was performed on DNA from all the samples to identify Blastocystis STs, amplifying a fragment of SSUrDNA and the ITS region. The amplicons were purified and sequenced, and consensus sequences were submitted to GenBank; afterwards, SSUrDNA sequences w

SUBMITTER: Villalobos G 

PROVIDER: S-EPMC4203911 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature