A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.

ABSTRACT: Matrix vesicles (MVs) play an important role in the initial stage of the process of bone mineralization, and are involved in multiple rare skeletal diseases with pathological mineralization or calcification. The aim of the study was to compare the proteomic profiling of osteoblast-like cells with and without mineralization ability (Saos-2 and U2-OS), and to identify novel mineralization-associated MV proteins. MVs were extracted using ExoQuick solution from mineralization-induced Saos-2 and U2-OS cells, and then were validated by transmission electron microscopy. A label-free quantitative proteomic method was used to compare the protein profiling of MVs from Saos-2 and U2-OS cells. Western-blots were used to confirm the expression of MVs proteins identified in proteomic studies. In our pro

SUBMITTER: Jiang L 

PROVIDER: S-EPMC4204581 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature