Characterization of human mesothelin transcripts in ovarian and pancreatic cancer.

ABSTRACT:

Background

Mesothelin is an attractive target for cancer immunotherapy due to its restricted expression in normal tissues and high level expression in several tumor types including ovarian and pancreatic adenocarcinomas. Three mesothelin transcript variants have been reported, but their relative expression in normal tissues and tumors has been poorly characterized. The goal of the present study was to clarify which mesothelin transcript variants are commonly expressed in human tumors.

Methods

Human genomic and EST nucleotide sequences in the public databases were used to evaluate sequences reported for the three mesothelin transcript variants in silico. Subsequently, RNA samples from normal ovary, ovarian and pancreatic carcinoma cell lines, and primary ovarian tumors were a

SUBMITTER: Muminova ZE 

PROVIDER: S-EPMC420467 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature