Comparative characterization of GmSPX members reveals that GmSPX3 is involved in phosphate homeostasis in soybean.

ABSTRACT:

Background and aims

Proteins containing the SPX (SYG1/Pho81/XPR1) domain are vital components in the phosphorus (P) signalling pathway, and regulate phosphate (Pi) homeostasis in plants. However, the characteristics and functions of GmSPX members in soybean (Glycine max) remain largely unknown.

Methods

BLAST searching revealed nine GmSPX members in the soybean genome. Subsequently, expression patterns of GmSPX members were investigated in various tissues of soybean grown in nutrient solution or sand culture through quantitative real-time PCR (qPCR) analysis. Sub-cellular localization of GmSPX was examined via transient expression of 35S:GmSPX-GFP in epidermal cells of onion (Allium cepa). Finally, soybean transgenic composite plants were generated to study GmSPX3 functions.<

SUBMITTER: Yao Z 

PROVIDER: S-EPMC4204674 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature