A high performance, cost-effective, open-source microscope for scanning two-photon microscopy that is modular and readily adaptable.

ABSTRACT: Two-photon laser scanning microscopy has revolutionized the ability to delineate cellular and physiological function in acutely isolated tissue and in vivo. However, there exist barriers for many laboratories to acquire two-photon microscopes. Additionally, if owned, typical systems are difficult to modify to rapidly evolving methodologies. A potential solution to these problems is to enable scientists to build their own high-performance and adaptable system by overcoming a resource insufficiency. Here we present a detailed hardware resource and protocol for building an upright, highly modular and adaptable two-photon laser scanning fluorescence microscope that can be used for in vitro or in vivo applications. The microscope is comprised of high-end componentry on a skeleton of off-the-she

SUBMITTER: Rosenegger DG 

PROVIDER: S-EPMC4204885 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature