Escherichia coli SeqA structures relocalize abruptly upon termination of origin sequestration during multifork DNA replication.

ABSTRACT: The Escherichia coli SeqA protein forms complexes with new, hemimethylated DNA behind replication forks and is important for successful replication during rapid growth. Here, E. coli cells with two simultaneously replicating chromosomes (multifork DNA replication) and YFP tagged SeqA protein was studied. Fluorescence microscopy showed that in the beginning of the cell cycle cells contained a single focus at midcell. The focus was found to remain relatively immobile at midcell for a period of time equivalent to the duration of origin sequestration. Then, two abrupt relocalization events occurred within 2-6 minutes and resulted in SeqA foci localized at each of the cell's quarter positions. Imaging of cells containing an additional fluorescent tag in the origin region showed that SeqA coloca

SUBMITTER: Fossum-Raunehaug S 

PROVIDER: S-EPMC4204900 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature