Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes.

ABSTRACT: Short peptide loops selected from phage libraries can specifically recognize the formation of hapten-antibody immunocomplexes and can thus be used to develop phage anti-immunocomplex assays (PHAIA) for noncompetitive detection of small molecules. In this study, we generated recombinant chimeras by fusing anti-immunocomplex peptides selected from phage libraries to the N- or C-termini of core streptavidin and used them to setup phage-free noncompetitive assays for the herbicide clomazone (MW 240 Da). The best conditions for refolding were optimized by a high throughput screening allowing to obtain tens of mg of purified protein per liter of culture. The noncompetitive assay developed with these chimeras performed with a 50% saturating concentration (SC50) of 2.2 ± 0.3 ng/mL and limit of det

SUBMITTER: Carlomagno M 

PROVIDER: S-EPMC4204917 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature