Association between SREBP-1 gene expression in mammary gland and milk fat yield in Sarda breed sheep.

ABSTRACT: The aim of this study was to examine the expression patterns of SREBP-1 gene in milk somatic cells and its association with milk fat yield during early lactation in Sarda breed sheep. A sample of 20 Sarda ewes, aged between 4 and 5 years, in their third to fourth lactation were chosen. From each ewe 28 days after lambing milk yield was measured, and a 160 ml milk sample for the RNA extraction and to test somatic cells count and lactose, fat and protein contents were collected. From the obtained RNA, total cDNA was synthesized and the quantitative PCR was performed. The fat, proteins and lactose content showed many differences among the animals, but these variations were no correlated with the milk yield. The SREBP-1 gene expression resulted higher in the high milk fat producing ewes. The c

SUBMITTER: Carcangiu V 

PROVIDER: S-EPMC4205032 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature