Quantitative analysis of tumor mitochondrial RNA using microarray.

ABSTRACT:

Aim

To design a novel method to rapidly detect the quantitative alteration of mtRNA in patients with tumors.

Methods

Oligo 6.22 and Primer Premier 5.0 bio-soft were used to design 15 pairs of primers of mtRNA cDNA probes in light of the functional and structural property of mtDNA, and then RT-PCR amplification was used to produce 15 probes of mtRNA from one normal gastric mucosal tissue. Total RNA extracted from 9 gastric cancers and corresponding normal gastric mucosal tissues was reverse transcribed into cDNA labeled with fluorescein. The spotted mtDNA microarrays were made and hybridized. Finally, the microarrays were scanned with a GeneTAC laser scanner to get the hybridized results. Northern blot was used to confirm the microarray results.

Results

The hybridized

SUBMITTER: Han CB 

PROVIDER: S-EPMC4205380 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature