A signaling network stimulated by β2 integrin promotes the polarization of lytic granules in cytotoxic cells.

ABSTRACT: Cytotoxic lymphocytes kill target cells through the polarized release of the contents of intracellular perforin-containing granules. In natural killer (NK) cells, the binding of β2 integrin to members of the intercellular adhesion molecule family is sufficient to promote not only the adhesion of NK cells to target cells but also the polarization of intracellular lytic granules toward the target. We used NK cells in an experimental system designed to enable us to study the polarization of lytic granules in the absence of their release through degranulation, as well as β2 integrin signaling independently of inside-out signals from other receptors. Through a proteomics approach, we identified a signaling network centered on an integrin-linked kinase (ILK)-Pyk2-paxillin core that was required

SUBMITTER: Zhang M 

PROVIDER: S-EPMC4205566 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature