A 3D matrix platform for the rapid generation of therapeutic anti-human carcinoma monoclonal antibodies.

ABSTRACT: Efforts to develop unbiased screens for identifying novel function-blocking monoclonal antibodies (mAbs) in human carcinomatous states have been hampered by the limited ability to design in vitro models that recapitulate tumor cell behavior in vivo. Given that only invasive carcinoma cells gain permanent access to type I collagen-rich interstitial tissues, an experimental platform was established in which human breast cancer cells were embedded in 3D aldimine cross-linked collagen matrices and used as an immunogen to generate mAb libraries. In turn, cancer-cell-reactive antibodies were screened for their ability to block carcinoma cell proliferation within collagen hydrogels that mimic the in vivo environment. As a proof of principle, a single function-blocking mAb out of 15 identified was

SUBMITTER: Dudley DT 

PROVIDER: S-EPMC4205646 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature