ATP-based chemotherapy response assay in primary or recurrent ovarian and peritoneal cancer.

ABSTRACT:

Purpose

To investigate chemosensitivity with an adenosine triphosphate-based chemotherapy response assay in patients with epithelial ovarian or peritoneal cancer according to tumor histology, grade, and disease status.

Materials and methods

One hundred specimens were collected during primary or secondary debulking from 67 patients with primary ovarian cancer, 24 patients with recurrent ovarian cancer, 5 patients with primary peritoneal cancer, and 4 patients with recurrent peritoneal cancer; samples were collected between August 2006 and June 2009. Tumor cells were isolated and cultured for 48 hours in media containing chemotherapy. The chemosensitivity index (CI) was calculated as 300 minus the sum of the cell death rate at 0.2×, 1×, and 5× drug concentrations, and the CI v

SUBMITTER: Lee M 

PROVIDER: S-EPMC4205709 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature