MRNA concentrations of MIF in subcutaneous abdominal adipose cells are associated with adipocyte size and insulin action.

ABSTRACT:

Objective

To determine whether the mRNA concentrations of inflammation response genes in isolated adipocytes and in cultured preadipocytes are related to adipocyte size and in vivo insulin action in obese individuals.

Design

Cross-sectional inpatient study.

Subjects

Obese Pima Indians with normal glucose tolerance.

Measurements

Adipocyte diameter (by microscope technique; n=29), expression of candidate genes (by quantitative real-time PCR) in freshly isolated adipocytes (monocyte chemoattractant protein (MCP) 1 and MCP2, macrophage inflammatory protein (MIP) 1alpha, MIP1beta and MIP2, macrophage migration inhibitory factor (MIF), tumor necrosis factor alpha, interleukin (IL) 6 and IL8; n=22) and cultured preadipocytes (MCP1, MIP1alpha, MIF, IL6 and matrix metal

SUBMITTER: Koska J 

PROVIDER: S-EPMC4205943 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature