Development of a heat-shock inducible gene expression system in the red alga Cyanidioschyzon merolae.

ABSTRACT: The cell of the unicellular red alga Cyanidioschyzon merolae contains a single chloroplast and mitochondrion, the division of which is tightly synchronized by a light/dark cycle. The genome content is extremely simple, with a low level of genetic redundancy, in photosynthetic eukaryotes. In addition, transient transformation and stable transformation by homologous recombination have been reported. However, for molecular genetic analyses of phenomena that are essential for cellular growth and survival, inducible gene expression/suppression systems are needed. Here, we report the development of a heat-shock inducible gene expression system in C. merolae. CMJ101C, encoding a small heat shock protein, is transcribed only when cells are exposed to an elevated temperature. Using a superfolder GF

SUBMITTER: Sumiya N 

PROVIDER: S-EPMC4206486 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature