Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides.

ABSTRACT: Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.

SUBMITTER: Yamazoe S 

PROVIDER: S-EPMC4206551 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature