Metabolic engineering of Methylobacterium extorquens AM1 for 1-butanol production.

ABSTRACT:

Background

Butanol is a promising next generation fuel and a bulk chemical precursor. Although clostridia are the primary industrial microbes for the fermentative production of 1-butanol, alternative engineered hosts have the potential to generate 1-butanol from alternative carbon feedstocks via synthetic metabolic pathways. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating products such as 1-butanol from one-carbon and two-carbon feedstocks. Moreover, the core methylotrophic pathways in M. extorquens AM1 involve unusual coenzyme A (CoA)-derivative metabolites, such as crotonyl-CoA, which is a precursor for the production of 1-butanol.

Results

In this work, we engineered a modified C

SUBMITTER: Hu B 

PROVIDER: S-EPMC4207312 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature