Multiplexed digital mRNA profiling of the inflammatory response in the West Nile Swiss Webster mouse model.

ABSTRACT:

Background and purpose

The ability to track changes in gene expression following viral infection is paramount to understanding viral pathogenesis. This study was undertaken to evaluate the nCounter, a high throughput digital gene expression system, as a means to better understand West Nile virus (WNV) dissemination and the inflammatory response against WNV in the outbred Swiss Webster (SW) mouse model over the course of infection.

Methodology

The nCounter Mouse Inflammation gene expression kit containing 179 inflammation related genes was used to analyze gene expression changes in multiple tissues over a nine day course of infection in SW mice following intraperitoneal injection with WNV. Protein expression levels for a subset of these cytokine/chemokine genes were determine

SUBMITTER: Pena J 

PROVIDER: S-EPMC4207670 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature