In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection.

ABSTRACT:

Purpose

To define global transcriptional responses of Staphylococcus aureus and its codY mutant (CodY is a transcription regulator of virulence and metabolic genes in response to branched-chain amino acids) when growing in bovine aqueous (AH) and vitreous humor (VH) in vitro, and to investigate the impact of codY deletion on S. aureus virulence in a novel murine anterior chamber (AC) infection model.

Methods

For the in vitro model, differential transcriptomic gene expression of S. aureus and its codY mutant grown in chemically defined medium (CDM), AH, and VH was analyzed. Furthermore, the strains were inoculated into the AC of mice. Changes in bacterial growth, electroretinography and inflammation scores were monitored.

Results

Bovine AH and VH provide sufficient nut

SUBMITTER: Sadaka A 

PROVIDER: S-EPMC4207797 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature