Reference gene validation for quantitative RT-PCR during biotic and abiotic stresses in Vitis vinifera.

ABSTRACT: Grapevine is one of the most cultivated fruit crop worldwide with Vitis vinifera being the species with the highest economical importance. Being highly susceptible to fungal pathogens and increasingly affected by environmental factors, it has become an important agricultural research area, where gene expression analysis plays a fundamental role. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is currently amongst the most powerful techniques to perform gene expression studies. Nevertheless, accurate gene expression quantification strongly relies on appropriate reference gene selection for sample normalization. Concerning V. vinifera, limited information still exists as for which genes are the most suitable to be used as reference under particular experimental conditi

SUBMITTER: Borges AF 

PROVIDER: S-EPMC4207820 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature