Strain prioritization for natural product discovery by a high-throughput real-time PCR method.

ABSTRACT: Natural products offer unmatched chemical and structural diversity compared to other small-molecule libraries, but traditional natural product discovery programs are not sustainable, demanding too much time, effort, and resources. Here we report a strain prioritization method for natural product discovery. Central to the method is the application of real-time PCR, targeting genes characteristic to the biosynthetic machinery of natural products with distinct scaffolds in a high-throughput format. The practicality and effectiveness of the method were showcased by prioritizing 1911 actinomycete strains for diterpenoid discovery. A total of 488 potential diterpenoid producers were identified, among which six were confirmed as platensimycin and platencin dual producers and one as a viguiepinol

SUBMITTER: Hindra 

PROVIDER: S-EPMC4208669 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature