Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.

ABSTRACT:

Objectives

To assess the role of coronary vasa vasorum (VV) spatial distribution in determining the location of early atherosclerotic lesion development.

Methods and results

Six, 3-month-old, female, crossbred swine were fed 2% high-cholesterol (HC) diet for 3 months prior to euthanasia. Six other pigs were fed normal diet (N) for the entire 6 months. Right coronary arteries were harvested and scanned intact with micro-CT (20mum cubic-voxel-size). After scanning, randomly selected cross-sectional histological sections were stained for nuclear-factor kappaB (NF-kappaB), hypoxia-inducible factor-1alpha (HIF-1alpha), macrophages, von-Willebrand-factor, dihydroethidium (DHE), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). The number of positive stained cells,

SUBMITTER: Gossl M 

PROVIDER: S-EPMC4208719 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature