Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model.

ABSTRACT: Mesenchymal stem cells (MSCs) possess unique paracrine and immunosuppressive properties, which make them useful candidates for cellular therapy. Here, we address how cellular senescence influences the therapeutic potential of human MSCs (hMSCs). Senescence was induced in bone marrow-derived hMSC cultures with gamma irradiation. Control and senescent cells were tested for their immunoregulatory activity in vitro and in vivo, and an extensive molecular characterization of the phenotypic changes induced by senescence was performed. We also compared the gene expression profiles of senescent hMSCs with a collection of hMSCs used in an ongoing clinical study of Graft Versus Host disease (GVHD). Our results show that senescence induces extensive phenotypic changes in hMSCs and abrogates their pro

SUBMITTER: Sepulveda JC 

PROVIDER: S-EPMC4209016 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature