MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines.

ABSTRACT:

Aim

To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.

Methods

An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.

Results

Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the c

SUBMITTER: Hummel R 

PROVIDER: S-EPMC4209553 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature