Modular, multi-input transcriptional logic gating with orthogonal LacI/GalR family chimeras.

ABSTRACT: In prokaryotes, the construction of synthetic, multi-input promoters is constrained by the number of transcription factors that can simultaneously regulate a single promoter. This fundamental engineering constraint is an obstacle to synthetic biologists because it limits the computational capacity of engineered gene circuits. Here, we demonstrate that complex multi-input transcriptional logic gating can be achieved through the use of ligand-inducible chimeric transcription factors assembled from the LacI/GalR family. These modular chimeras each contain a ligand-binding domain and a DNA-binding domain, both of which are chosen from a library of possibilities. When two or more chimeras have the same DNA-binding domain, they independently and simultaneously regulate any promoter containing th

SUBMITTER: Shis DL 

PROVIDER: S-EPMC4210161 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature