Multivariate biophysical markers predictive of mesenchymal stromal cell multipotency.

ABSTRACT: The capacity to produce therapeutically relevant quantities of multipotent mesenchymal stromal cells (MSCs) via in vitro culture is a common prerequisite for stem cell-based therapies. Although culture expanded MSCs are widely studied and considered for therapeutic applications, it has remained challenging to identify a unique set of characteristics that enables robust identification and isolation of the multipotent stem cells. New means to describe and separate this rare cell type and its downstream progenitor cells within heterogeneous cell populations will contribute significantly to basic biological understanding and can potentially improve efficacy of stem and progenitor cell-based therapies. Here, we use multivariate biophysical analysis of culture-expanded, bone marrow-derived MSCs,

SUBMITTER: Lee WC 

PROVIDER: S-EPMC4210311 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature