AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation.

ABSTRACT:

Background

Follistatin (FST) has been shown to bind to some TGF-β family members and can function as a potent myostatin (MSTN) antagonist. Recent studies have revealed that over-expression of FST by adeno-associated viruses increases muscle growth in mice, humans and nonhuman primates. In the present study, to determine the effect of FST on ovine primary myoblast (OPM) proliferation, FST was over-expressed using an adeno-associated virus serotype 2 (AAV 2) vector.

Results

Western blot results showed that AAV induced the expression of FST protein in transduced OPM cells. Real-time quantitative PCR results indicated that over-expression of FST resulted in a dramatic increase in Akt I and CDK2 expression and a decrease in p21 expression. Moreover, cell cycle analysis confirmed

SUBMITTER: Nazari M 

PROVIDER: S-EPMC4210504 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature