Astragalus saponins modulates colon cancer development by regulating calpain-mediated glucose-regulated protein expression.

ABSTRACT:

Background

Glucose-regulated proteins (GRP) are induced in the cancer microenvironment to promote tumor survival, metastasis and drug resistance. AST was obtained from the medicinal plant Astragalus membranaceus, which possesses anti-tumor and pro-apoptotic properties in colon cancer cells and tumor xenograft. The present study aimed to investigate the involvement of GRP in endoplasmic reticulum (ER) stress-mediated apoptosis during colon cancer development, with focus on the correlation between AST-evoked regulation of GRP and calpain activation.

Methods

The effects of AST on GRP and apoptotic activity were assessed in HCT 116 human colon adenocarcinoma cells. Calpain activity was examined by using a fluorescence assay kit. Immunofluorescence staining and immunoprecipitatio

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4210535 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature