Extensive stage-regulation of translation revealed by ribosome profiling of Trypanosoma brucei.

ABSTRACT:

Background

Trypanosoma brucei subspecies infect humans and animals in sub-Saharan Africa. This early diverging eukaryote shows many novel features in basic biological processes, including the use of polycistronic transcription to generate all protein-coding mRNAs. Therefore we hypothesized that translational control provides a means to tune gene expression during parasite development in mammalian and fly hosts.

Results

We used ribosome profiling to examine genome-wide protein synthesis in animal-derived slender bloodstream forms and cultured procyclic (insect midgut) forms. About one-third of all CDSs showed statistically significant regulation of protein production between the two stages. Of these, more than two-thirds showed a change in translation efficiency, but few appe

SUBMITTER: Jensen BC 

PROVIDER: S-EPMC4210626 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature