The orphan protein bis-γ-glutamylcystine reductase joins the pyridine nucleotide disulfide reductase family.

ABSTRACT: Facile DNA sequencing became possible decades after many enzymes had been purified and characterized. Consequently, there are still "orphan" enyzmes for which activities are known but for which encoding genes have not been identified. Identification of the genes encoding orphan enzymes is important because it allows correct annotation of genes of unknown function or with misassigned function. Bis-γ-glutamylcystine reductase (GCR) is an orphan protein that was purified in 1988. This enzyme catalyzes the reduction of bis-γ-glutamylcystine. γ-Glutamylcysteine is the major low-molecular weight thiol in halobacteria. We purified GCR from Halobacterium sp. NRC-1 and identified the sequence of 23 tryptic peptides by nano-liquid chromatography electrospray ionization tandem mass spectrometry. Thes

SUBMITTER: Kim J 

PROVIDER: S-EPMC4211611 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature