Katanin p60 promotes neurite growth and collateral formation in the hippocampus.

ABSTRACT:

Objective

This study aimed to investigate the effect of Katanin p60 on the neurite growth and collateral formation in the hippocampus.

Methods

Gene cloning was performed to construct the Katanin p60 eukaryotic vector. The microtubule cutting effect and protein expression of Katanin p60 were investigated in 293T cells. Then, these vectors were transfected into hippocampal neurons of rats, and the effects of Katanin p60 on the neurite growth and collateral formation were observed.

Results

In the present study, we successfully constructed Katanin p60-GFP recombinant plasmids. After transfecting into 293T cells, the Katanin p60 was over-expressed in these cells, the mesh-like structure of microtubules was disrupted, the residual microtubules circled the nucleus, the expre

SUBMITTER: Chen K 

PROVIDER: S-EPMC4211748 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature