GSTP1 arrests bladder cancer T24 cells in G0/G1 phase and up-regulates p21 expression.

ABSTRACT:

Objective

GSTP1 over-expression was introduced into human bladder cancer T24 cells via the lentivirus system. The influence of GSTP1 on the proliferation and cell cycle of T24 cells as well as the potential mechanisms was investigated.

Methods

The lentiviral vector GSTP1-pWPXL was constructed and transfected into T24 cells in the presence of Lipofectamine 2000. CCK8 assay and colony formation test were performed to explore the impact of GSTP1 on the proliferation of T24 cells. Ollowing PI staining, flow cytometry was done to detect the proportion of T24 cells in different phases. Western blot assay was conducted to detect the protein p21 expression.

Results

When compared with control group, T24 cells with GSTP1 over-expression showed significant reduction in cell prol

SUBMITTER: Gao L 

PROVIDER: S-EPMC4211822 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature