Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction.

ABSTRACT:

Objective

To determine interrelationships between the expression of long intergenic (noncoding) RNA-p21 (lincRNA-p21), NF-κB activity, and responses to methotrexate (MTX) in rheumatoid arthritis (RA) by analyzing patient blood samples and cell culture models.

Methods

Expression levels of long noncoding RNA and messenger RNA (mRNA) were determined by quantitative reverse transcription-polymerase chain reaction. Western blotting and flow cytometry were used to quantify levels of intracellular proteins. Intracellular NF-κB activity was determined using an NF-κB luciferase reporter plasmid.

Results

Patients with RA expressed reduced basal levels of lincRNA-p21 and increased basal levels of phosphorylated p65 (RelA), a marker of NF-κB activation. Patients with RA who were

SUBMITTER: Spurlock CF 

PROVIDER: S-EPMC4211976 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature