Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF.

ABSTRACT: Upregulated expression of nucleolar GTPase nucleostemin (NS) has been associated with increased cellular proliferation potential and tumor malignancy during cancer development. Recent reports attribute the growth regulatory effects of NS protein to its role in facilitating ribosome production. However, the oncogenic potential of NS remains unclear, as imbalanced levels of NS have been reported to exert growth inhibitory effect by modulating p53 tumor-suppressor activity. It also remains in questions if aberrant NS levels might have a p53-independent role in regulation of cell proliferation and growth. In this study, we performed affinity purification and mass spectrometry analysis to explore protein-protein interactions influencing NS growth regulatory properties independently of p53 tumor

SUBMITTER: Lo D 

PROVIDER: S-EPMC4212020 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature