Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel.

ABSTRACT: Engineered cardiac tissues have been constructed with primary or stem cell-derived cardiac cells on natural or synthetic scaffolds. They represent a tremendous potential for the treatment of injured areas through the addition of tensional support and delivery of sufficient cells. In this study, 1-6 million (M) neonatal cardiac cells were seeded on fibrin gels to fabricate cardiac tissue patches, and the effects of culture time and cell density on spontaneous contraction rates, twitch forces and paced response frequencies were measured. Electrocardiograms and signal volume index of connexin 43 were also analysed. Patches of 1-6 M cell densities exhibited maximal contraction rates in the range 305-410 beats/min (bpm) within the first 4 days after plating; low cell density (1-3 M) patches sus

SUBMITTER: Tao ZW 

PROVIDER: S-EPMC4212052 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature