In-house ELISA screening using a locally-isolated Leptospira in Malaysia: determination of its cut-off points.

ABSTRACT:

Background

Leptospirosis is a zoonotic disease caused by Leptospira species and is distributed globally. Microscopic agglutination test (MAT) is the serological 'gold standard' for diagnosis of leptospirosis but it is time-consuming and labour-intensive. An alternative serological method that is rapid, sensitive and specific is important for early treatment to reduce morbidity and mortality. The use of local Leptospira isolation may improve the sensitivity and specificity of the test because it may varies from one geographical region to another region. The objective of this study was to determine the sensitivity, specificity and cut-off points for an in-house Immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) using a locally isolated Leptospiral strain IMR/175 as the

SUBMITTER: Tan XT 

PROVIDER: S-EPMC4212092 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature