A simple fluorescence labeling method for studies of protein oxidation, protein modification, and proteolysis.

ABSTRACT: Proteins are sensitive to oxidation, and oxidized proteins are excellent substrates for degradation by proteolytic enzymes such as the proteasome and the mitochondrial Lon protease. Protein labeling is required for studies of protein turnover. Unfortunately, most labeling techniques involve (3)H or (14)C methylation, which is expensive, exposes researchers to radioactivity, generates large amounts of radioactive waste, and allows only single-point assays because samples require acid precipitation. Alternative labeling methods have largely proven unsuitable, either because the probe itself is modified by the oxidant(s) being studied or because the alternative labeling techniques are too complex or too costly for routine use. What is needed is a simple, quick, and cheap labeling technique th

SUBMITTER: Pickering AM 

PROVIDER: S-EPMC4212338 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature