Isolation of salt stress-related genes from Aspergillus glaucus CCHA by random overexpression in Escherichia coli.

ABSTRACT: The halotolerant fungus Aspergillus glaucus CCHA was isolated from the surface of wild vegetation around a saltern with the salinity range being 0-31%. Here, a full-length cDNA library of A. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. Among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and transport, or regulation of transcription. Subsequently, the cDNA library was transformed into E. coli cells to screen for putative salt stress-related clones. Five putative positive clones were obtained from E. coli cells grown on LB agar containing 1 M NaCl, on which they showed rapid growth compared

SUBMITTER: Fang J 

PROVIDER: S-EPMC4212599 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature