AAV-mediated targeting of gene expression to the peri-infarct region in rat cortical stroke model.

ABSTRACT:

Background

For stroke patients the recovery of cognitive and behavioral functions is often incomplete. Functional recovery is thought to be mediated largely by connectivity rearrangements in the peri-infarct region. A method for manipulating gene expression in this region would be useful for identifying new recovery-enhancing treatments.

New method

We have characterized a way of targeting adeno-associated virus (AAV) vectors to the peri-infarct region of cortical ischemic lesion in rats 2days after middle cerebral artery occlusion (MCAo).

Results

We used magnetic resonance imaging (MRI) to show that the altered properties of post-ischemic brain tissue facilitate the spreading of intrastriatally injected nanoparticles toward the infarct. We show that subcortical inject

SUBMITTER: Matlik K 

PROVIDER: S-EPMC4212894 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature