High throughput identification of monoclonal antibodies to membrane bound and secreted proteins using yeast and phage display.

ABSTRACT: Antibodies are ubiquitous and essential reagents for biomedical research. Uses of antibodies include quantifying proteins, identifying the temporal and spatial pattern of expression in cells and tissue, and determining how proteins function under normal or pathological conditions. Specific antibodies are only available for a small portion of the proteome, limiting study of those proteins for which antibodies do not exist. The technologies to generate target-specific antibodies need to be improved to obtain high quality antibodies to the proteome at reasonable cost. Here we show that renewable, validated, and standardized monoclonal antibodies can be generated at high throughput, without the need for antigen production or animal immunizations. In this study, 60 protein domains from 24 selec

SUBMITTER: Zhao L 

PROVIDER: S-EPMC4213037 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature