Palmitoylethanolamide regulates development of intestinal radiation injury in a mast cell-dependent manner.

ABSTRACT:

Background

Mast cells and neuroimmune interactions regulate the severity of intestinal radiation mucositis, a dose-limiting toxicity during radiation therapy of abdominal malignancies.

Aim

Because endocannabinoids (eCB) regulate intestinal inflammation, we investigated the effect of the cannabimimetic, palmitoylethanolamide (PEA), in a mast competent (+/+) and mast cell-deficient (Ws/Ws) rat model.

Methods

Rats underwent localized, fractionated intestinal irradiation, and received daily injections with vehicle or PEA from 1 day before until 2 weeks after radiation. Intestinal injury was assessed noninvasively by luminol bioluminescence, and, at 2 weeks, by histology, morphometry, and immunohistochemical analysis, gene expression analysis, and pathway analysis.

Resu

SUBMITTER: Wang J 

PROVIDER: S-EPMC4213290 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature