Transport by populations of fast and slow kinesins uncovers novel family-dependent motor characteristics important for in vivo function.

ABSTRACT: Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challenging and requires tight coupling between experiments and modeling to uncover the underlying motor behavior. To understand how diverse kinesins attached to the same cargo coordinate their movement, we carried out microtubule gliding assays using pairwise mixtures of motors from the kinesin-1, -2, -3, -5, and -7 families engineered to have identical run lengths and surface attachments. Uniform motor densities were used and microtubule gliding speeds were measured for varying prop

SUBMITTER: Arpag G 

PROVIDER: S-EPMC4213720 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature