Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).

ABSTRACT: Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly stable housekeeping gene (HKG) as an internal control is recommended and necessary. However, there are several reports suggesting that regulation of some HKGs is affected by different conditions. The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States and Europe. The expression profile of target genes related to insecticide exposure, resistance, and RNA interference has become an important experimental technique for study of western corn rootworms; however, lack of information on reliable HKGs under different conditions makes the interpretation

SUBMITTER: Rodrigues TB 

PROVIDER: S-EPMC4214676 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature