Transfusion of older stored blood worsens outcomes in canines depending on the presence and severity of pneumonia.

ABSTRACT:

Background

In experimental pneumonia we found that transfused older blood increased mortality and lung injury that was associated with increased in vivo hemolysis and elevated plasma cell-free hemoglobin (CFH), non-transferrin-bound iron (NTBI), and plasma labile iron (PLI) levels. In this study, we additionally analyze identically treated animals that received lower or higher bacterial doses.

Study design and methods

Two-year-old purpose-bred beagles (n = 48) challenged intrabronchially with Staphylococcus aureus (0 [n = 8], 1.0 × 10(9) [n = 8], 1.25 × 10(9) [n = 24], and ≥1.5 × 10(9) [n = 8] colony-forming units/kg) were exchange transfused with either 7- or 42-day-old canine universal donor blood (80 mL/kg in four divided doses).

Results

The greater increases in CF

SUBMITTER: Wang D 

PROVIDER: S-EPMC4214924 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature